Aminoacetonitrile이 백서발육치근막에 미치는 영향에 관한 실험적 연구
EFFECT OF AMINOACETONITRILE ON THE DEVELOPING RAT PERIDONTAL MEMBRANE
소속 상세정보
강민선/Min Sun Kang
KMID : 0358919780050010033
Abstract
저자는 aminoacetonitrile을 임신 백서에다 투여하고 그 영향이 자백서의 치근막 헝성에 미
친 바를 생후 1일, 7일, 14일 및 21일 경과된 경우의 치근막에서 조직 및 조직화학적으로 검
색된 바가 있다.
치근막에 대한 상기 검색방법은, H-E염색과 결합조직섬유성분에 대한 van Gieson,
Masson's trichrom, argyrophilic 및 oxytalan 섬유에 대한 Halmi씨 염색법 methyl
green-pyronic염색 및 PAS반응법을 실시하여 본 바에서 다음과 같은 결과를 얻었다.
1. 교원섬유의 기능적 배열이 지연되는 소견이 생후 1일의 자백에서 관찰되었으나 생후 7
일경과예 이후부터는 점차 회복되는 조직소견이었다.
2. 은호성섬유의 베열은 생후 1일과 7일에서는 섬세하고 소성으로 배열되고 있으나 생후
14일경과예 이후부터는 점차 굵어지고 치밀하여지는 소견이었다.
3. Oxytalan fiber의 배열은 생후 1일경과예에서는 분지상으로 짧게 주행되고 있으나 14일
경과예 이후부터는 수적으로도 증가하고 치근중간위에서 치경부높이의 치조골벽을 향해 사
주하는 섭유가 관찰되었다.
4. 섬유아세포에서의 pyronin호성은 생후 7일이후부터 점진적으로 증염되는 경향이었고
PAS양성물질은 생후 21일경과예에서, 치경부에서 치근중간위에 이르는 치근표층부위에서
관찰되었다.
It has been studied that aminoacetonitrile was associated with the inhibition of collagen
fiber, argyrophilic fiber and oxytalan fiber synthesis. This experiment was performed, by
the basic knowledge of above mentioned study, to study on the biological effect of
aminoacetonitrile to the developing periodontal ligament in Sprague Dawley rat. twenty
two of female rats weighing about 200gm were gestated.
In 7 days after gestation, the experimental rats were injected aminoacetonitrile 7 times
intraperitoncally.
After parturition, delivered fetuses were divided into 4 groups and each group was
sacrificed to 1 day, 7 days, 14 days, and 21 days after delivery, schematically. All the
fetuses were observed on their periodontal ligament by histological and histochemical
methods.
To study on the components of periodontal ligament fiber in these experimental study
van Gieson, Masson's trichrom, argyrophilic fiber, oxytalan fiber, methyl green pyronin
and periodic acid-Shiff staining were performed.
Results were as follows;
1) Retardation of functional orientation in periodontal ligament collagen fiber was
observed in 1 day fetuses but this appearance was diminished gradually and recovered
in normal condition in 7 days fetuses.
2) Distribution of argyrophilic fiber in 1 day fetuses was oriented delicately and loosely
but volume of this fiber was gradually thickened arid distributed densely.
3) Oxytalan fiber was oriented dendritically and contradictorily in 14 days fetuses but
their orientation was changed into oblique form in middle portion of roof and their
numbers were increased gradually.
4) Pyronin-philic stain of fibroblast wag gradually deepened in 7 days fetuses and this
finding also suggested to the depreciation of collagen synthesis in this specimen.
5) PAS positive line was observed continuousely at the portion of cervical to the
middle root surface.
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